Construct: shRNA TRCN0000116181

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182790.1-281s1c1
DNA Barcode:
GAAGCCAAAGATGTCTACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NAMPT (10135)
Gene Description:
nicotinamide phosphoribosyltransferase
Transcript:
RefSeq NM_182790.1 (NON-CURRENT)
Match location:
Position 281 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116181)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCCAAAGATGTCTACAAA
Hairpin Sequence:
5'-CCGG-GAAGCCAAAGATGTCTACAAA-CTCGAG-TTTGTAGACATCTTTGGCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCCAAAGATGTCTACAAACTCGAGTTTGTAGACATCTTTGGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCCAAAGATGTCTACAAACTCGAGTTTGTAGACATCTTTGGCTTC-3'

Other clones with same target sequence:

(none)