Construct: shRNA TRCN0000116266

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080812.2-2481s1c1
DNA Barcode:
GAAGATGGCTTACCAGTCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COL13A1 (1305)
Gene Description:
collagen type XIII alpha 1 chain
Transcript:
RefSeq NM_080812.2 (NON-CURRENT)
Match location:
Position 2481 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116266)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGATGGCTTACCAGTCCAA
Hairpin Sequence:
5'-CCGG-GAAGATGGCTTACCAGTCCAA-CTCGAG-TTGGACTGGTAAGCCATCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGATGGCTTACCAGTCCAACTCGAGTTGGACTGGTAAGCCATCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGATGGCTTACCAGTCCAACTCGAGTTGGACTGGTAAGCCATCTTC-3'

Other clones with same target sequence:

(none)