Construct: shRNA TRCN0000116312

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006136.2-2040s1c1
DNA Barcode:
CCTGTGGTGAATGTTAGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAPZA2 (830)
Gene Description:
capping actin protein of muscle Z-line subunit alpha 2
Transcript:
RefSeq NM_006136.2 (NON-CURRENT)
Match location:
Position 2040 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116312)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGGTGAATGTTAGTATT
Hairpin Sequence:
5'-CCGG-CCTGTGGTGAATGTTAGTATT-CTCGAG-AATACTAACATTCACCACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGGTGAATGTTAGTATTCTCGAGAATACTAACATTCACCACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGGTGAATGTTAGTATTCTCGAGAATACTAACATTCACCACAGG-3'

Other clones with same target sequence:

(none)