Construct: shRNA TRCN0000116322

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001376.2-14002s1c1
DNA Barcode:
CCTTTCTGTAATAGTGAAAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DYNC1H1 (1778)
Gene Description:
dynein cytoplasmic 1 heavy chain 1
Transcript:
RefSeq NM_001376.2 (NON-CURRENT)
Match location:
Position 14002 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116322)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTCTGTAATAGTGAAAGT
Hairpin Sequence:
5'-CCGG-CCTTTCTGTAATAGTGAAAGT-CTCGAG-ACTTTCACTATTACAGAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTCTGTAATAGTGAAAGTCTCGAGACTTTCACTATTACAGAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTCTGTAATAGTGAAAGTCTCGAGACTTTCACTATTACAGAAAGG-3'

Other clones with same target sequence:

(none)