Construct: shRNA TRCN0000116339

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_498222.1-86s1c1
DNA Barcode:
CGGAAGAGATGCAACAGGACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC442331 (442331)
Gene Description:
similar to dynein, cytoplasmic, light peptide; 8kD LC; dynein LC8; protein inhibitor of neuronal nitric oxide synthase
Transcript:
RefSeq XM_498222.1 (NON-CURRENT)
Match location:
Position 86 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116339)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAAGAGATGCAACAGGACT
Hairpin Sequence:
5'-CCGG-CGGAAGAGATGCAACAGGACT-CTCGAG-AGTCCTGTTGCATCTCTTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAAGAGATGCAACAGGACTCTCGAGAGTCCTGTTGCATCTCTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAAGAGATGCAACAGGACTCTCGAGAGTCCTGTTGCATCTCTTCCG-3'

Other clones with same target sequence:

(none)