Construct: shRNA TRCN0000116382

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032518.2-2503s1c1
DNA Barcode:
GCCTGATTTAAGGAATCAGGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COL25A1 (84570)
Gene Description:
collagen type XXV alpha 1 chain
Transcript:
RefSeq NM_032518.2 (NON-CURRENT)
Match location:
Position 2503 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116382)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGATTTAAGGAATCAGGT
Hairpin Sequence:
5'-CCGG-GCCTGATTTAAGGAATCAGGT-CTCGAG-ACCTGATTCCTTAAATCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGATTTAAGGAATCAGGTCTCGAGACCTGATTCCTTAAATCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGATTTAAGGAATCAGGTCTCGAGACCTGATTCCTTAAATCAGGC-3'

Other clones with same target sequence:

(none)