Construct: shRNA TRCN0000116394

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005382.1-1225s1c1
DNA Barcode:
GAGACTAGATTTAGCACATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEFM (4741)
Gene Description:
neurofilament medium
Transcript:
RefSeq NM_005382.1 (NON-CURRENT)
Match location:
Position 1225 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116394)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGACTAGATTTAGCACATTT
Hairpin Sequence:
5'-CCGG-GAGACTAGATTTAGCACATTT-CTCGAG-AAATGTGCTAAATCTAGTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGACTAGATTTAGCACATTTCTCGAGAAATGTGCTAAATCTAGTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGACTAGATTTAGCACATTTCTCGAGAAATGTGCTAAATCTAGTCTC-3'

Other clones with same target sequence:

(none)