Construct: shRNA TRCN0000116425

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020441.2-169s1c1
DNA Barcode:
GTCAAGAACGACCAGTGCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CORO1B (57175)
Gene Description:
coronin 1B
Transcript:
RefSeq NM_020441.2 (NON-CURRENT)
Match location:
Position 169 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116425)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAAGAACGACCAGTGCTAT
Hairpin Sequence:
5'-CCGG-GTCAAGAACGACCAGTGCTAT-CTCGAG-ATAGCACTGGTCGTTCTTGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAAGAACGACCAGTGCTATCTCGAGATAGCACTGGTCGTTCTTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAAGAACGACCAGTGCTATCTCGAGATAGCACTGGTCGTTCTTGAC-3'

Other clones with same target sequence:

  1. TRCN0000289724