Construct: shRNA TRCN0000116465

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005550.2-2428s1c1
DNA Barcode:
GACGCTCTATTCCCTCAAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIFC3 (3801)
Gene Description:
kinesin family member C3
Transcript:
RefSeq NM_005550.2 (NON-CURRENT)
Match location:
Position 2428 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116465)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGCTCTATTCCCTCAAGTT
Hairpin Sequence:
5'-CCGG-GACGCTCTATTCCCTCAAGTT-CTCGAG-AACTTGAGGGAATAGAGCGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGCTCTATTCCCTCAAGTTCTCGAGAACTTGAGGGAATAGAGCGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGCTCTATTCCCTCAAGTTCTCGAGAACTTGAGGGAATAGAGCGTC-3'

Other clones with same target sequence:

(none)