Construct: shRNA TRCN0000116472

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012156.2-3294s1c1
DNA Barcode:
CCCACTTGAATGCAAAGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPB41L1 (2036)
Gene Description:
erythrocyte membrane protein band 4.1 like 1
Transcript:
RefSeq NM_012156.2 (CURRENT)
Match location:
Position 3294 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116472)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACTTGAATGCAAAGGAAA
Hairpin Sequence:
5'-CCGG-CCCACTTGAATGCAAAGGAAA-CTCGAG-TTTCCTTTGCATTCAAGTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACTTGAATGCAAAGGAAACTCGAGTTTCCTTTGCATTCAAGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACTTGAATGCAAAGGAAACTCGAGTTTCCTTTGCATTCAAGTGGG-3'

Other clones with same target sequence:

  1. TRCN0000286998