Construct: shRNA TRCN0000116592
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001013660.1-2498s1c1
- DNA Barcode:
- CCAGCCTTTAGAGAACAACAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FRRS1 (391059)
- Gene Description:
- ferric chelate reductase 1
- Transcript:
- RefSeq NM_001013660.1 (NON-CURRENT)
- Match location:
- Position 2498 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000116592)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCAGCCTTTAGAGAACAACAT
- Hairpin Sequence:
-
5'-CCGG-CCAGCCTTTAGAGAACAACAT-CTCGAG-ATGTTGTTCTCTAAAGGCTGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCAGCCTTTAGAGAACAACATCTCGAGATGTTGTTCTCTAAAGGCTGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCAGCCTTTAGAGAACAACATCTCGAGATGTTGTTCTCTAAAGGCTGG-3'
Other clones with same target sequence:
(none)
