Construct: shRNA TRCN0000116621

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032010.1-918s1c1
DNA Barcode:
GCCTGGAATAAACAGCATGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP1B (4131)
Gene Description:
microtubule associated protein 1B
Transcript:
RefSeq NM_032010.1 (NON-CURRENT)
Match location:
Position 918 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116621)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGGAATAAACAGCATGTT
Hairpin Sequence:
5'-CCGG-GCCTGGAATAAACAGCATGTT-CTCGAG-AACATGCTGTTTATTCCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGGAATAAACAGCATGTTCTCGAGAACATGCTGTTTATTCCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGGAATAAACAGCATGTTCTCGAGAACATGCTGTTTATTCCAGGC-3'

Other clones with same target sequence:

  1. TRCN0000307232