Construct: shRNA TRCN0000116699

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138424.1-1085s1c1
DNA Barcode:
CGGAACCTGTACGGGATGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIF12 (113220)
Gene Description:
kinesin family member 12
Transcript:
RefSeq NM_138424.1 (CURRENT)
Match location:
Position 1085 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116699)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAACCTGTACGGGATGCTA
Hairpin Sequence:
5'-CCGG-CGGAACCTGTACGGGATGCTA-CTCGAG-TAGCATCCCGTACAGGTTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAACCTGTACGGGATGCTACTCGAGTAGCATCCCGTACAGGTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAACCTGTACGGGATGCTACTCGAGTAGCATCCCGTACAGGTTCCG-3'

Other clones with same target sequence:

(none)