Construct: shRNA TRCN0000116719

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012307.2-1143s1c1
DNA Barcode:
CCGGGAGAGTTTGAACAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPB41L3 (23136)
Gene Description:
erythrocyte membrane protein band 4.1 like 3
Transcript:
RefSeq NM_012307.2 (NON-CURRENT)
Match location:
Position 1143 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116719)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGGAGAGTTTGAACAATTT
Hairpin Sequence:
5'-CCGG-CCGGGAGAGTTTGAACAATTT-CTCGAG-AAATTGTTCAAACTCTCCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGGAGAGTTTGAACAATTTCTCGAGAAATTGTTCAAACTCTCCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGGAGAGTTTGAACAATTTCTCGAGAAATTGTTCAAACTCTCCCGG-3'

Other clones with same target sequence:

(none)