Construct: shRNA TRCN0000116778

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080747.1-811s1c1
DNA Barcode:
CCTTGACAGATGAGATTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KRT72 (140807)
Gene Description:
keratin 72
Transcript:
RefSeq NM_080747.1 (NON-CURRENT)
Match location:
Position 811 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116778)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGACAGATGAGATTAAAT
Hairpin Sequence:
5'-CCGG-CCTTGACAGATGAGATTAAAT-CTCGAG-ATTTAATCTCATCTGTCAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGACAGATGAGATTAAATCTCGAGATTTAATCTCATCTGTCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGACAGATGAGATTAAATCTCGAGATTTAATCTCATCTGTCAAGG-3'

Other clones with same target sequence:

(none)