Construct: shRNA TRCN0000116850

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001849.2-3097s1c1
DNA Barcode:
CGGCTTCTTCGACCGCTTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COL6A2 (1292)
Gene Description:
collagen type VI alpha 2 chain
Transcript:
RefSeq NM_001849.2 (NON-CURRENT)
Match location:
Position 3097 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116850)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCTTCTTCGACCGCTTCAT
Hairpin Sequence:
5'-CCGG-CGGCTTCTTCGACCGCTTCAT-CTCGAG-ATGAAGCGGTCGAAGAAGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCTTCTTCGACCGCTTCATCTCGAGATGAAGCGGTCGAAGAAGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCTTCTTCGACCGCTTCATCTCGAGATGAAGCGGTCGAAGAAGCCG-3'

Other clones with same target sequence:

(none)