Construct: shRNA TRCN0000116881
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_006870.3-502s1c1
- DNA Barcode:
- GCTTTGTATGATGCAAGCTTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DSTN (11034)
- Gene Description:
- destrin, actin depolymerizing factor
- Transcript:
- RefSeq NM_006870.3 (NON-CURRENT)
- Match location:
- Position 502 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000116881)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTTTGTATGATGCAAGCTTT
- Hairpin Sequence:
-
5'-CCGG-GCTTTGTATGATGCAAGCTTT-CTCGAG-AAAGCTTGCATCATACAAAGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTTTGTATGATGCAAGCTTTCTCGAGAAAGCTTGCATCATACAAAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTTTGTATGATGCAAGCTTTCTCGAGAAAGCTTGCATCATACAAAGC-3'
Other clones with same target sequence:
-
TRCN0000310417
