Construct: shRNA TRCN0000116902

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003389.2-2094s1c1
DNA Barcode:
GCCCATGATGAGCTGTAACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CORO2A (7464)
Gene Description:
coronin 2A
Transcript:
RefSeq NM_003389.2 (NON-CURRENT)
Match location:
Position 2094 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116902)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATGATGAGCTGTAACTT
Hairpin Sequence:
5'-CCGG-GCCCATGATGAGCTGTAACTT-CTCGAG-AAGTTACAGCTCATCATGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATGATGAGCTGTAACTTCTCGAGAAGTTACAGCTCATCATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATGATGAGCTGTAACTTCTCGAGAAGTTACAGCTCATCATGGGC-3'

Other clones with same target sequence:

  1. TRCN0000299219