Construct: shRNA TRCN0000116904

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003389.2-1502s1c1
DNA Barcode:
GAGACCTATCTTCAATTCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CORO2A (7464)
Gene Description:
coronin 2A
Transcript:
RefSeq NM_003389.2 (NON-CURRENT)
Match location:
Position 1502 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116904)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGACCTATCTTCAATTCCAT
Hairpin Sequence:
5'-CCGG-GAGACCTATCTTCAATTCCAT-CTCGAG-ATGGAATTGAAGATAGGTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGACCTATCTTCAATTCCATCTCGAGATGGAATTGAAGATAGGTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGACCTATCTTCAATTCCATCTCGAGATGGAATTGAAGATAGGTCTC-3'

Other clones with same target sequence:

  1. TRCN0000333690