Construct: shRNA TRCN0000116946

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021647.5-483s1c1
DNA Barcode:
GTGTGTGGCTTCTAACATCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MFAP3L (9848)
Gene Description:
microfibril associated protein 3 like
Transcript:
RefSeq NM_021647.5 (NON-CURRENT)
Match location:
Position 483 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116946)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTGTGGCTTCTAACATCTA
Hairpin Sequence:
5'-CCGG-GTGTGTGGCTTCTAACATCTA-CTCGAG-TAGATGTTAGAAGCCACACAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTGTGGCTTCTAACATCTACTCGAGTAGATGTTAGAAGCCACACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTGTGGCTTCTAACATCTACTCGAGTAGATGTTAGAAGCCACACAC-3'

Other clones with same target sequence:

(none)