Construct: shRNA TRCN0000116966

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000501.1-400s1c1
DNA Barcode:
GCCGGAGTGAAGCCTGGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ELN (2006)
Gene Description:
elastin
Transcript:
RefSeq NM_000501.1 (NON-CURRENT)
Match location:
Position 400 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116966)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGGAGTGAAGCCTGGGAAA
Hairpin Sequence:
5'-CCGG-GCCGGAGTGAAGCCTGGGAAA-CTCGAG-TTTCCCAGGCTTCACTCCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGGAGTGAAGCCTGGGAAACTCGAGTTTCCCAGGCTTCACTCCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGGAGTGAAGCCTGGGAAACTCGAGTTTCCCAGGCTTCACTCCGGC-3'

Other clones with same target sequence:

(none)