Construct: shRNA TRCN0000116981

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006108.1-1417s1c1
DNA Barcode:
CAGAGTTGTCATCGAGAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPON1 (10418)
Gene Description:
spondin 1
Transcript:
RefSeq NM_006108.1 (NON-CURRENT)
Match location:
Position 1417 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000116981)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAGTTGTCATCGAGAGAAT
Hairpin Sequence:
5'-CCGG-CAGAGTTGTCATCGAGAGAAT-CTCGAG-ATTCTCTCGATGACAACTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAGTTGTCATCGAGAGAATCTCGAGATTCTCTCGATGACAACTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAGTTGTCATCGAGAGAATCTCGAGATTCTCTCGATGACAACTCTG-3'

Other clones with same target sequence:

  1. TRCN0000300421