Construct: shRNA TRCN0000117006

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004321.4-3952s1c1
DNA Barcode:
CCGCTTATATCGAGATGGAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIF1A (547)
Gene Description:
kinesin family member 1A
Transcript:
RefSeq NM_004321.4 (NON-CURRENT)
Match location:
Position 3952 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000117006)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCTTATATCGAGATGGAGA
Hairpin Sequence:
5'-CCGG-CCGCTTATATCGAGATGGAGA-CTCGAG-TCTCCATCTCGATATAAGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCTTATATCGAGATGGAGACTCGAGTCTCCATCTCGATATAAGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCTTATATCGAGATGGAGACTCGAGTCTCCATCTCGATATAAGCGG-3'

Other clones with same target sequence:

(none)