Construct: shRNA TRCN0000117047

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015719.2-5788s1c1
DNA Barcode:
CCTTTCTACTTCCACGGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COL5A3 (50509)
Gene Description:
collagen type V alpha 3 chain
Transcript:
RefSeq NM_015719.2 (NON-CURRENT)
Match location:
Position 5788 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000117047)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTCTACTTCCACGGTGAA
Hairpin Sequence:
5'-CCGG-CCTTTCTACTTCCACGGTGAA-CTCGAG-TTCACCGTGGAAGTAGAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTCTACTTCCACGGTGAACTCGAGTTCACCGTGGAAGTAGAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTCTACTTCCACGGTGAACTCGAGTTCACCGTGGAAGTAGAAAGG-3'

Other clones with same target sequence:

(none)