Construct: shRNA TRCN0000117137

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004140.3-3567s1c1
DNA Barcode:
CCCTCACTTTGCAGAGTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LLGL1 (3996)
Gene Description:
LLGL scribble cell polarity complex component 1
Transcript:
RefSeq NM_004140.3 (NON-CURRENT)
Match location:
Position 3567 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000117137)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCACTTTGCAGAGTATTT
Hairpin Sequence:
5'-CCGG-CCCTCACTTTGCAGAGTATTT-CTCGAG-AAATACTCTGCAAAGTGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCACTTTGCAGAGTATTTCTCGAGAAATACTCTGCAAAGTGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCACTTTGCAGAGTATTTCTCGAGAAATACTCTGCAAAGTGAGGG-3'

Other clones with same target sequence:

(none)