Construct: shRNA TRCN0000117161

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173086.2-1297s1c1
DNA Barcode:
CCTCAAGGATGCTAAGAACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KRT6C (286887)
Gene Description:
keratin 6C
Transcript:
RefSeq NM_173086.2 (NON-CURRENT)
Match location:
Position 1297 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000117161)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAAGGATGCTAAGAACAA
Hairpin Sequence:
5'-CCGG-CCTCAAGGATGCTAAGAACAA-CTCGAG-TTGTTCTTAGCATCCTTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAAGGATGCTAAGAACAACTCGAGTTGTTCTTAGCATCCTTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAAGGATGCTAAGAACAACTCGAGTTGTTCTTAGCATCCTTGAGG-3'

Other clones with same target sequence:

(none)