Construct: shRNA TRCN0000117167

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_053177.7-1116s1c1
DNA Barcode:
CGCACTATCCAGTTTGTGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TUBA3E (112714)
Gene Description:
tubulin alpha 3e
Transcript:
RefSeq XM_053177.7 (NON-CURRENT)
Match location:
Position 1116 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000117167)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCACTATCCAGTTTGTGGAT
Hairpin Sequence:
5'-CCGG-CGCACTATCCAGTTTGTGGAT-CTCGAG-ATCCACAAACTGGATAGTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCACTATCCAGTTTGTGGATCTCGAGATCCACAAACTGGATAGTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCACTATCCAGTTTGTGGATCTCGAGATCCACAAACTGGATAGTGCG-3'

Other clones with same target sequence:

(none)