Construct: shRNA TRCN0000117205

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001017421.1-1123s1c1
DNA Barcode:
CCCTCCATTGTCCACCGCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POTEKP (440915)
Gene Description:
POTE ankyrin domain family member K, pseudogene
Transcript:
RefSeq NM_001017421.1 (NON-CURRENT)
Match location:
Position 1123 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000117205)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCCATTGTCCACCGCAAA
Hairpin Sequence:
5'-CCGG-CCCTCCATTGTCCACCGCAAA-CTCGAG-TTTGCGGTGGACAATGGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCCATTGTCCACCGCAAACTCGAGTTTGCGGTGGACAATGGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCCATTGTCCACCGCAAACTCGAGTTTGCGGTGGACAATGGAGGG-3'

Other clones with same target sequence:

(none)