Construct: shRNA TRCN0000117241

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014970.2-1033s1c1
DNA Barcode:
CCAAACCTTGAGAAAGGATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIFAP3 (22920)
Gene Description:
kinesin associated protein 3
Transcript:
RefSeq NM_014970.2 (NON-CURRENT)
Match location:
Position 1033 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000117241)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAACCTTGAGAAAGGATTA
Hairpin Sequence:
5'-CCGG-CCAAACCTTGAGAAAGGATTA-CTCGAG-TAATCCTTTCTCAAGGTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAACCTTGAGAAAGGATTACTCGAGTAATCCTTTCTCAAGGTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAACCTTGAGAAAGGATTACTCGAGTAATCCTTTCTCAAGGTTTGG-3'

Other clones with same target sequence:

(none)