Construct: shRNA TRCN0000117311

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_058242.1-557s1c1
DNA Barcode:
CAGCAGAACAAGGTTCTGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KRT6C (140446)
Gene Description:
keratin 6C
Transcript:
RefSeq NM_058242.1 (NON-CURRENT)
Match location:
Position 557 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000117311)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCAGAACAAGGTTCTGGAA
Hairpin Sequence:
5'-CCGG-CAGCAGAACAAGGTTCTGGAA-CTCGAG-TTCCAGAACCTTGTTCTGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCAGAACAAGGTTCTGGAACTCGAGTTCCAGAACCTTGTTCTGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCAGAACAAGGTTCTGGAACTCGAGTTCCAGAACCTTGTTCTGCTG-3'

Other clones with same target sequence:

(none)