Construct: shRNA TRCN0000117439

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_203302.2-770s1c1
DNA Barcode:
CCACCCTGATTTGTCCTGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPL23AP82 (284942)
Gene Description:
ribosomal protein L23a pseudogene 82
Transcript:
RefSeq NM_203302.2 (NON-CURRENT)
Match location:
Position 770 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000117439)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCCTGATTTGTCCTGATA
Hairpin Sequence:
5'-CCGG-CCACCCTGATTTGTCCTGATA-CTCGAG-TATCAGGACAAATCAGGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCCTGATTTGTCCTGATACTCGAGTATCAGGACAAATCAGGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCCTGATTTGTCCTGATACTCGAGTATCAGGACAAATCAGGGTGG-3'

Other clones with same target sequence:

(none)