Construct: shRNA TRCN0000117519

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_380072.1-235s1c1
DNA Barcode:
CACAATGTCAAGGAGCTGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPL32P17 (402716)
Gene Description:
ribosomal protein L32 pseudogene 17
Transcript:
RefSeq XM_380072.1 (NON-CURRENT)
Match location:
Position 235 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000117519)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACAATGTCAAGGAGCTGGAA
Hairpin Sequence:
5'-CCGG-CACAATGTCAAGGAGCTGGAA-CTCGAG-TTCCAGCTCCTTGACATTGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACAATGTCAAGGAGCTGGAACTCGAGTTCCAGCTCCTTGACATTGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACAATGTCAAGGAGCTGGAACTCGAGTTCCAGCTCCTTGACATTGTG-3'

Other clones with same target sequence:

(none)