Construct: shRNA TRCN0000117653

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052969.1-437s1c1
DNA Barcode:
CAGATGAAACCTGGTAGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPL39L (116832)
Gene Description:
ribosomal protein L39 like
Transcript:
RefSeq NM_052969.1 (NON-CURRENT)
Match location:
Position 437 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000117653)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGATGAAACCTGGTAGTAAA
Hairpin Sequence:
5'-CCGG-CAGATGAAACCTGGTAGTAAA-CTCGAG-TTTACTACCAGGTTTCATCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGATGAAACCTGGTAGTAAACTCGAGTTTACTACCAGGTTTCATCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGATGAAACCTGGTAGTAAACTCGAGTTTACTACCAGGTTTCATCTG-3'

Other clones with same target sequence:

  1. TRCN0000333228