Construct: shRNA TRCN0000117724

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014303.2-270s1c1
DNA Barcode:
CTTATCAAAGACATCAGGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PES1 (23481)
Gene Description:
pescadillo ribosomal biogenesis factor 1
Transcript:
RefSeq NM_014303.2 (NON-CURRENT)
Match location:
Position 270 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000117724)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTATCAAAGACATCAGGTTT
Hairpin Sequence:
5'-CCGG-CTTATCAAAGACATCAGGTTT-CTCGAG-AAACCTGATGTCTTTGATAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTATCAAAGACATCAGGTTTCTCGAGAAACCTGATGTCTTTGATAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTATCAAAGACATCAGGTTTCTCGAGAAACCTGATGTCTTTGATAAG-3'

Other clones with same target sequence:

  1. TRCN0000300706