Construct: shRNA TRCN0000117736

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_495936.1-736s1c1
DNA Barcode:
GTGACTGGAAGGGCCTGAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLIC1P1 (390363)
Gene Description:
chloride intracellular channel 1 pseudogene 1
Transcript:
RefSeq XM_495936.1 (NON-CURRENT)
Match location:
Position 736 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000117736)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGACTGGAAGGGCCTGAGTA
Hairpin Sequence:
5'-CCGG-GTGACTGGAAGGGCCTGAGTA-CTCGAG-TACTCAGGCCCTTCCAGTCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGACTGGAAGGGCCTGAGTACTCGAGTACTCAGGCCCTTCCAGTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGACTGGAAGGGCCTGAGTACTCGAGTACTCAGGCCCTTCCAGTCAC-3'

Other clones with same target sequence:

(none)