Construct: shRNA TRCN0000117838

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005669.3-224s1c1
DNA Barcode:
CCAGCCTACATCTCAATTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
REEP5 (7905)
Gene Description:
receptor accessory protein 5
Transcript:
RefSeq NM_005669.3 (NON-CURRENT)
Match location:
Position 224 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000117838)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCCTACATCTCAATTAAA
Hairpin Sequence:
5'-CCGG-CCAGCCTACATCTCAATTAAA-CTCGAG-TTTAATTGAGATGTAGGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCCTACATCTCAATTAAACTCGAGTTTAATTGAGATGTAGGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCCTACATCTCAATTAAACTCGAGTTTAATTGAGATGTAGGCTGG-3'

Other clones with same target sequence:

  1. TRCN0000300827