Construct: shRNA TRCN0000117934

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014708.3-6350s1c1
DNA Barcode:
GCCAGGCAGTATATCCAGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KNTC1 (9735)
Gene Description:
kinetochore associated 1
Transcript:
RefSeq NM_014708.3 (NON-CURRENT)
Match location:
Position 6350 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000117934)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGGCAGTATATCCAGTTA
Hairpin Sequence:
5'-CCGG-GCCAGGCAGTATATCCAGTTA-CTCGAG-TAACTGGATATACTGCCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGGCAGTATATCCAGTTACTCGAGTAACTGGATATACTGCCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGGCAGTATATCCAGTTACTCGAGTAACTGGATATACTGCCTGGC-3'

Other clones with same target sequence:

(none)