Construct: shRNA TRCN0000117936

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014708.3-4655s1c1
DNA Barcode:
GCCCTTGAGATGGTTCCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KNTC1 (9735)
Gene Description:
kinetochore associated 1
Transcript:
RefSeq NM_014708.3 (NON-CURRENT)
Match location:
Position 4655 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000117936)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTTGAGATGGTTCCTTTA
Hairpin Sequence:
5'-CCGG-GCCCTTGAGATGGTTCCTTTA-CTCGAG-TAAAGGAACCATCTCAAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTTGAGATGGTTCCTTTACTCGAGTAAAGGAACCATCTCAAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTTGAGATGGTTCCTTTACTCGAGTAAAGGAACCATCTCAAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000299301