Construct: shRNA TRCN0000117962

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_057177.4-2734s1c1
DNA Barcode:
GCGTGAAAGGATTGGAGCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PARD3B (117583)
Gene Description:
par-3 family cell polarity regulator beta
Transcript:
RefSeq NM_057177.4 (NON-CURRENT)
Match location:
Position 2734 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000117962)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTGAAAGGATTGGAGCAAA
Hairpin Sequence:
5'-CCGG-GCGTGAAAGGATTGGAGCAAA-CTCGAG-TTTGCTCCAATCCTTTCACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTGAAAGGATTGGAGCAAACTCGAGTTTGCTCCAATCCTTTCACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTGAAAGGATTGGAGCAAACTCGAGTTTGCTCCAATCCTTTCACGC-3'

Other clones with same target sequence:

(none)