Construct: shRNA TRCN0000118012

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174930.2-460s1c1
DNA Barcode:
CCATAAGGAATTTCAAAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PMS2P5 (5383)
Gene Description:
PMS1 homolog 2, mismatch repair system component pseudogene 5
Transcript:
RefSeq NM_174930.2 (NON-CURRENT)
Match location:
Position 460 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000118012)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATAAGGAATTTCAAAGGAA
Hairpin Sequence:
5'-CCGG-CCATAAGGAATTTCAAAGGAA-CTCGAG-TTCCTTTGAAATTCCTTATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATAAGGAATTTCAAAGGAACTCGAGTTCCTTTGAAATTCCTTATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATAAGGAATTTCAAAGGAACTCGAGTTCCTTTGAAATTCCTTATGG-3'

Other clones with same target sequence:

(none)