Construct: shRNA TRCN0000118047

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052821.3-1213s1c1
DNA Barcode:
GCCTCCTCTCTGAAGATGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDR5 (11091)
Gene Description:
WD repeat domain 5
Transcript:
RefSeq NM_052821.3 (CURRENT)
Match location:
Position 1213 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000118047)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCCTCTCTGAAGATGATT
Hairpin Sequence:
5'-CCGG-GCCTCCTCTCTGAAGATGATT-CTCGAG-AATCATCTTCAGAGAGGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCCTCTCTGAAGATGATTCTCGAGAATCATCTTCAGAGAGGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCCTCTCTGAAGATGATTCTCGAGAATCATCTTCAGAGAGGAGGC-3'

Other clones with same target sequence:

(none)