Construct: shRNA TRCN0000118091

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004392.4-1542s1c1
DNA Barcode:
GCTGTTGAAAGTTGCCATAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DACH1 (1602)
Gene Description:
dachshund family transcription factor 1
Transcript:
RefSeq NM_004392.4 (NON-CURRENT)
Match location:
Position 1542 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000118091)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTTGAAAGTTGCCATAGA
Hairpin Sequence:
5'-CCGG-GCTGTTGAAAGTTGCCATAGA-CTCGAG-TCTATGGCAACTTTCAACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTTGAAAGTTGCCATAGACTCGAGTCTATGGCAACTTTCAACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTTGAAAGTTGCCATAGACTCGAGTCTATGGCAACTTTCAACAGC-3'

Other clones with same target sequence:

(none)