Construct: shRNA TRCN0000118134

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019619.2-2782s1c1
DNA Barcode:
GCCATCGACAAATCTTATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PARD3 (56288)
Gene Description:
par-3 family cell polarity regulator
Transcript:
RefSeq NM_019619.2 (NON-CURRENT)
Match location:
Position 2782 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000118134)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATCGACAAATCTTATGAT
Hairpin Sequence:
5'-CCGG-GCCATCGACAAATCTTATGAT-CTCGAG-ATCATAAGATTTGTCGATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCGACAAATCTTATGATCTCGAGATCATAAGATTTGTCGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCGACAAATCTTATGATCTCGAGATCATAAGATTTGTCGATGGC-3'

Other clones with same target sequence:

  1. TRCN0000307858