Construct: shRNA TRCN0000118238

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032258.1-992s1c1
DNA Barcode:
GCCGATAACAAGAATCGCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBC1D3F (84218)
Gene Description:
TBC1 domain family member 3F
Transcript:
RefSeq NM_032258.1 (NON-CURRENT)
Match location:
Position 992 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000118238)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGATAACAAGAATCGCCTT
Hairpin Sequence:
5'-CCGG-GCCGATAACAAGAATCGCCTT-CTCGAG-AAGGCGATTCTTGTTATCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGATAACAAGAATCGCCTTCTCGAGAAGGCGATTCTTGTTATCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGATAACAAGAATCGCCTTCTCGAGAAGGCGATTCTTGTTATCGGC-3'

Other clones with same target sequence:

(none)