Construct: shRNA TRCN0000118262

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001851.1-2539s1c1
DNA Barcode:
GCACATAGAAACTCTCAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ITIH5 (80760)
Gene Description:
inter-alpha-trypsin inhibitor heavy chain 5
Transcript:
RefSeq NM_001001851.1 (NON-CURRENT)
Match location:
Position 2539 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000118262)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACATAGAAACTCTCAGAAA
Hairpin Sequence:
5'-CCGG-GCACATAGAAACTCTCAGAAA-CTCGAG-TTTCTGAGAGTTTCTATGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACATAGAAACTCTCAGAAACTCGAGTTTCTGAGAGTTTCTATGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACATAGAAACTCTCAGAAACTCGAGTTTCTGAGAGTTTCTATGTGC-3'

Other clones with same target sequence:

(none)