Construct: shRNA TRCN0000118343

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016129.2-647s1c1
DNA Barcode:
CGAGCATCGTTGCTTCAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COPS4 (51138)
Gene Description:
COP9 signalosome subunit 4
Transcript:
RefSeq NM_016129.2 (NON-CURRENT)
Match location:
Position 647 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000118343)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGCATCGTTGCTTCAGAAT
Hairpin Sequence:
5'-CCGG-CGAGCATCGTTGCTTCAGAAT-CTCGAG-ATTCTGAAGCAACGATGCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGCATCGTTGCTTCAGAATCTCGAGATTCTGAAGCAACGATGCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGCATCGTTGCTTCAGAATCTCGAGATTCTGAAGCAACGATGCTCG-3'

Other clones with same target sequence:

(none)