Construct: shRNA TRCN0000118390

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016116.1-1076s1c1
DNA Barcode:
CCGATGATGACTTGGAGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ASB4 (51666)
Gene Description:
ankyrin repeat and SOCS box containing 4
Transcript:
RefSeq NM_016116.1 (NON-CURRENT)
Match location:
Position 1076 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000118390)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGATGATGACTTGGAGAAAT
Hairpin Sequence:
5'-CCGG-CCGATGATGACTTGGAGAAAT-CTCGAG-ATTTCTCCAAGTCATCATCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGATGATGACTTGGAGAAATCTCGAGATTTCTCCAAGTCATCATCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGATGATGACTTGGAGAAATCTCGAGATTTCTCCAAGTCATCATCGG-3'

Other clones with same target sequence:

(none)