Construct: shRNA TRCN0000118406

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153696.2-1392s1c1
DNA Barcode:
GTCGAGATTATGCTGTAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FOLH1B (219595)
Gene Description:
folate hydrolase 1B
Transcript:
RefSeq NM_153696.2 (CURRENT)
Match location:
Position 1392 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000118406)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCGAGATTATGCTGTAGTTT
Hairpin Sequence:
5'-CCGG-GTCGAGATTATGCTGTAGTTT-CTCGAG-AAACTACAGCATAATCTCGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCGAGATTATGCTGTAGTTTCTCGAGAAACTACAGCATAATCTCGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCGAGATTATGCTGTAGTTTCTCGAGAAACTACAGCATAATCTCGAC-3'

Other clones with same target sequence:

(none)