Construct: shRNA TRCN0000118437

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001005366.1-4269s1c1
DNA Barcode:
CGGCCTTTACAAGAAGACATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KDM2B (84678)
Gene Description:
lysine demethylase 2B
Transcript:
RefSeq NM_001005366.1 (NON-CURRENT)
Match location:
Position 4269 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000118437)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCCTTTACAAGAAGACATT
Hairpin Sequence:
5'-CCGG-CGGCCTTTACAAGAAGACATT-CTCGAG-AATGTCTTCTTGTAAAGGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCCTTTACAAGAAGACATTCTCGAGAATGTCTTCTTGTAAAGGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCCTTTACAAGAAGACATTCTCGAGAATGTCTTCTTGTAAAGGCCG-3'

Other clones with same target sequence:

(none)