Construct: shRNA TRCN0000118492

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207382.1-1794s1c1
DNA Barcode:
CGTAAGTAGATGGCTCTCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBE2Q2P1 (388165)
Gene Description:
ubiquitin conjugating enzyme E2 Q2 pseudogene 1
Transcript:
RefSeq NM_207382.1 (NON-CURRENT)
Match location:
Position 1794 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000118492)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTAAGTAGATGGCTCTCTTA
Hairpin Sequence:
5'-CCGG-CGTAAGTAGATGGCTCTCTTA-CTCGAG-TAAGAGAGCCATCTACTTACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTAAGTAGATGGCTCTCTTACTCGAGTAAGAGAGCCATCTACTTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTAAGTAGATGGCTCTCTTACTCGAGTAAGAGAGCCATCTACTTACG-3'

Other clones with same target sequence:

(none)